Lecture 22

1.
What are examples of immortalised cell lines (ICLs)?
Hela, SH-SY5Y, PC12 (rats)
2.
What are the advantages of IMCs?
Continuous supply of cells, limited variability, easy to culture/manipulate
3.
What are the disadvantages of ICLs?
Not real disease model, limited variability
4.
What are the disadvantages of primary cells?
Not all tissue types, limited life span
5.
Where do human embryonic stem cells come from?
Blastocye inner mass
6.
What are the advantages of HES?
Pluripotent, variability, genetic disease models
7.
What are induced pluripotent stem cells (iPSCs)?
Skin cells reprogrammed to pluripotent stem cells
8.
What are the disadvantages to iPSCs?
Long time to mature, low yield, variability between people
9.
What are human organoids?
3D organoids grown from SCs
10.
What are preclinical tests that can be added to drug development?
iPSC in vitro, iPSC of diseased and control