Lecture 29

1.
What is the term for hospital acquired infection in hospital?
Nosocomial
2.
When is an infection considered nosocomial?
"If symptoms appear 48 hours after admission, within 30 days after discharge"
3.
How might nosocomial infections occur?
"Surgery, catheter, gastrointestinal"
4.
What does wound infection rate depend upon?
"Quantity of contamination, virility of microbes, perfusion after surgery, foreign material in wound, host immunity, antimicrobial treatment"
5.
How might infection occur with skin cancer removal?
Bacteria in sweat glands or hair follicles transferred to deeper layers of skin with scapel (S. aureus) - 1-5% chance
6.
How might an appendectomy cause infection?
Intestinal contents contaminate open muscle/fat/skin. E. coli? 10-20%
7.
How might trauma cause infection?
"Organisms from skin, gravel, environmental objects piercing skin. S. aureus. 5-10%"
8.
How to prevent wound infection?
"Avoid contamination, cleaning to remove dead tissue, avoid creating fluid collections, antibiotics"
9.
When is the best time to give antibiotics for surgery?
1 hour before
10.
How can a catheter cause infection?
Bacteria colonising the urethral mietus transferred to bladder. E. coli 5% per day
11.
How might C. dificile cause infection?
Transfer of faeces onto fomites. 10% per week of hospitilisation
12.
How can predisposing factors cause nosocomial infections?
"Underlying illness insertion of foreign bodies, shared facilities"
13.
How can nosocomial infections be prevented?
"Sterilisation, disinfection, cleaning, isolation"
14.
What is sterile?
100% clean
15.
What is disinfection?
Mostly clean
16.
How does steam sterilise?
120 degress celcius at 100 Kpa
17.
Whow also can sterilisation be achieved?
Poisoness chemicals
18.
What temperature are most viruses killed?
60 degress celcius
19.
What temperature are most vegetative bacteria killed?
80 degress celcius
20.
What temperation are B. sterothermophilus spores killed? 
120 degress celcius
21.
What does disinfection not kill?
Bacterial spores
22.
What chemicals are used as disinfectants?
"Alcohols, H2O2"
23.
Which things need to be sterile?
Things going in sterile places
24.
Which  things need to be disinfected?
Things going into non sterile places
25.
Which things need to be cleaned?
Things that aren't going into the body
26.
How to inactivate HIV?
Wash surface with bleach
27.
What must be done with CJD contamination?
Discard instruments contaminated with brain tissue